XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Posters
78.
PRODUCTION OF 3HYDROXYPROPIONALDEHYD BY THE STraINS OF
LACTOBACILLUS reuTerI
Hana Kiňová Sepová, Andrea Bilková, František Bilka and Lýdia Bezáková
Department of Cell and Molecular Biology of Drugs FaF UK Bratislava
Eight bacterial strains were isolated from stomach mucus of breast fed lamb (C, D, and
E) and kid (KO4b, KO4m, KO5, KG1, KG4). Four of these strains were identified by sequencing
of 16S rDNA as Lactobacillus reuteri (E, KO4b, KO4m, and KO5) and tested for
production of antimicrobial substance 3hydroxypropionaldehyd (3HPA). For this purpose
PCR primers targeting gene of large subunit of glycerol dehydratase (GD), key enzyme
involved in 3HPA synthesis, were designed. Presence of this gene-fragment was detected
in the case of all four strains and the reference strain L. reuteri ATCC 55730. Sequencing
of PCR product proved that it is fragment of gene of large subunit of GD. The ability to
produce 3HPA was determined spectrophotometrically, positive reaction was noticed
in the case of L. reuteri E and reference strain. Quantative analysis of 3HPA production
by L. reuteri E and L. reuteri ATCC 55730 in aerobic and anaerobic conditions was determined
spectrophotometrically by tryptophan method. The amount of 3HPA produced
aerobically was in both strains higher than those produced anaerobically. In the case of
L. reuteri E it was aerobically 1.60 mmol/L, anaerobically 0.58 mmol/L and in the case of
L. reuteri ATCC 55730 it was aerobically 184.51 mmol/L and anaerobically 5.71 mmol/L.
Strain L. reuteri E produced lower amounts of 3-HPA than probiotic strain L. reuteri
ATCC 55730. However this does not exclude it from the group of potential probiotics;
it has other important attributes that allow L. reuteri E to be a good probiotic strain for
veterinary use.
XXII. Biochemistry Congress, Martin
201