XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Posters
68.
STUDY OF THE EffECT OF HISTONE DEACETYLASE INHIBITOR ON THE
SENSITIVITY OF LEUKAEMIC CELLS TO THE CYTOSTATICS
Andrea Štefániková 1 , Jozef Hatok 1 , Jana Jurečeková 1 , Ivana Plameňová 2 ,
Dušan Dobrota 1 and Peter Račay 1
1
Department of Medical Biochemistry, 2 Clinic of Haematology and Transfusiology,
JLF UK Martin
One of the possibilities how to overcome a chemoresistance of tumor cells is a combination
of classic cytostatics with substances possessing a potential to block proliferation
or induce apoptosis of these cells. Histone deacetylases are a group of enzymes that
catalyze removing acetic acid residue from histones. This epigenetic process leads to
condensation of chromatin and suppresion of transcription. The influence of histone
deacetylases inhibitors is intensively studied nowadays, especially because of their connection
with antiproliferative and antineoplastic effect. There has long been known an
inhibitor of histone deacetylases class I - sodium butyrate. It is a short chain fatty acid
that has effects at the molecular, cellular, and tissue level. By performing of in vitro MTT
test, we have revealed polyresistance of leukaemic blasts isolated from blood of AML
patient to almost all cytostatics tested. Addition of sodium butyrate in concentration of
2mM led to significantly increased chemosensitivity of blasts. In search for molecular
mechanism of butyrate action we have performed analysis of leukaemic cell line HL60
treated with sodium butyrate. Our results open possibility that addition of butyrate
increases chemosensitivity of blasts by alteration of expression of proteins involved in
apoptosis initiation.
Acknowledgement: This work was supported by grant UK/223/2010 to Andrea Štefaniková.
190 XXII. Biochemistry Congress, Martin