XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta
XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta
XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta
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Posters<br />
65.<br />
THE FUNCTIONALITY of aPOPTOSOME APParaTUs aND THE<br />
EXPRESSION OF ITS rEGULaTOrS IN NON-SMaLL CELL LUNG<br />
caRCINOMA<br />
Erika Moravčíková 1 , Evžen Křepela 1 , Jan Procházka 1 ,<br />
Jan Čermák 1 and Kamila Benková 2<br />
1<br />
Department of Pneumology and Thoracic Surgery, 2 Department of Pathology,<br />
University Hospital Bulovka, Prague, Czech Republic<br />
Deficient signaling in the apoptosome pathway may contribute to tumorigenesis and<br />
neoplastic progression of malignant tumors as well as to their chemo- and radioresistance.<br />
In the present study, we investigated the functionality of the apoptosome apparatus<br />
(AA), the expression of mRNAs encoding the activatable and inactivatable Apaf1<br />
protein variants, and the expression of AA regulators, including nucling/UACA, APIP, and<br />
procaspase-2 (PC-2) in human non-small cell lung carcinoma (NSCLC) cells and tissues.<br />
First, the enzymatic analysis showed that in 2 of 6 examined NSCLC cell lines and in 18<br />
of 59 NSCLC tissues (from surgically treated patients) the cytochrome-c (cyt-c) plus dATP<br />
could trigger a significant increase in cytosolic caspase-3-like activity. Furthermore, the<br />
endogenous as well as the (cyt-c + dATP)-induced caspase-3-like activities were higher<br />
in NSCLC tissues as compared to matched lungs. Both in the tumors and the lungs, the<br />
expression of mRNAs encoding the activatable Apaf1-XL and -LC variants was higher<br />
than the expression of mRNAs encoding the inactivatable Apaf1-LN and -S variants.<br />
Interestingly, the expression of both nucling/UACA and APIP was downregulated in<br />
NSCLC tissues as compared to the lungs, but it did not correlate with the AA activation<br />
in NSCLC cell lines and tissues and lungs. The expression of PC-2 mRNA in the tumors<br />
was higher as compared to the lungs, but there was no correlation between PC-2 mRNA<br />
and the endogenous caspase-3-like activity in NSCLC tissues. The results of this study<br />
indicate that the expression of nucling/UACA, APIP and PC-2 did not importantly affect<br />
the functionality of AA in NSCLC.<br />
Acknowledgments: Supported by research projects (MZO 00064211 and NS/9715-3) from<br />
Ministry of Health, Czech Republic.<br />
<strong>XXII</strong>. Biochemistry Congress, Martin<br />
187
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