XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Posters
64.
PROGNOSTIC SIGNIFICANCE OF MIr-21 AND MIr-143 EXPRESSION IN
TISSUE SAMPLES OF COLORECTAL CarCINOMA AND COLORECTAL
LIVER METASTASES
Vlastimil Kulda 1 , Martin Pešta 2 , Ondřej Topolčan 2 , Lukáš Řehoř 1 , Martin Svatoň 1 ,
Václav Liška 3 , Václav Babuška 1 , Luboš Holubec 2 and Radim Černý 1
1
Department of biochemistry LF UK Plzeň, 2 Department of internal medicine
II LF UK Plzeň, 3 Department of surgery LF UK Plzeň
Some of the microRNAs, which are the endogenously expressed regulatory small
noncoding RNA molecules, have an altered expression in colorectal cancer. The aim of
our study was to assess the relationship between miR-21 and miR-143 expression and
prognostic/clinicopathological features of colorectal carcinoma (CRC) and colorectal
liver metastases (CLM) as well. The estimation was performed in 46 paired (tumor and
control) tissue samples of CRC. Further we studied 30 tissue samples of CLM. MiR-21
and miR-143 expressions were quantified using the qRT-PCR method. Relation of miR-21
and miR-143 expression to DFI (disease free interval) (Wilcoxon; p=0.0026 and p=0.0191,
respectively) was recorded. There was shorter DFI in patients with a higher expression
of miR-21 and surprisingly also in patients with a higher expression of miR-143, which
is a putative tumor suppressor. There was a higher expression of miR-21 and lower
expression of miR-143 in CRC tissue in comparison with adjacent normal colon tissue
(p