XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Posters
60.
EffECT OF fraCTIONATED DOSES OF GAMMA raYS ON THE ROSTraL
MIGraTORY STreaM Of aDULT raTS
Soňa Bálentová 1 , Eva Hajtmanová 2 , Yvetta Mellová 3 ,
Ivana Kinclová 2 and Marián Adamkov 1
1
Institute of Histology and Embryology, Jessenius Faculty of Medicine in Martin,
CU, Martin, Slovakia, 2 Department of Radiotherapy and Oncology, Martin Faculty
Hospital, Martin, Slovakia, 3 Institute of Anatomy, Jessenius Faculty of Medicine
in Martin, CU, Martin, Slovakia
Ionizing radiation commonly used in the radiotherapy of brain tumours can cause adverse
side effects to surrounding normal brain tissue. The adult mammalian subventricular
zone (SVZ) of the brain lateral ventricles (LV) and their subsequent lateral ventricular
extension, the rostral migratory stream (RMS), is one of the few areas, which retains the
ability to generate new neurons and glial cells throughout life. Take into account the fact,
that ionizing radiation is one of the strongest exogenous factors affecting cell proliferation,
the aim of the present study was to investigate the occurence of radiation-induced
alterations of apoptosis in the forebrain’s RMS using animal model of radiosurgery. Adult
male Wistar rats were investigated 7, 14 or 21 days after whole-body irradiation with
fractionated doses of gamma rays (the total dose of 3 Gy). Radiation-induced apoptotic
cell death was determined by in situ labeling of DNA nick ends (TUNEL) and light microscopy
evaluation of TUNEL-positive cells. However, the data from quantitative analysis of
the numbers of apoptotic cells are still under evaluation, our preliminary results showed,
that ionizing radiation clearly affect this neurogenic region.
Acknowledgement: This work was supported by AV4/2026/08 Grant.
182 XXII. Biochemistry Congress, Martin