XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Posters
56.
EffECT OF PAMAM G4 DENDRIMER ON LIVER MITOCHONDRIA
OXIDATIVE PHOSPHORYLATION AND METABOLIC CONTROL IN
EXPERIMENTAL DIABETES
Oľga Vančová 1 , Oľga Uličná 1 , Katarína Šebeková 2 ,
Magdalena Labieniec 3 and Cezary Watala 3
1
Pharmacobiochemical laboratory 3rd Dept of Int Medicine LFUK Bratislava,
2
Department of Preventive and Clinical Medicine SZU Bratislava, 3 Department of
Haemostasis and Haemostatic Dis Med Univ of Lodz, Poland
Prolonged exposure to hyperglycaemia causes non-enzymatic glycation of proteins and
can lead to production of reactive oxygen species.
Polyamidoamine dendrimer PAMAM G4, a strong nucleophilic molecule with 64 free
primary amino groups at its surface appears as an effective scavenger of excessive
glucose in diabetes.
The aim was to study the ability of PAMAM G4 to lower the concentration of plasma
glucose, to suppress long-term parameters of hyperglycaemia and to improve oxidative
phosphorylation in the liver mitochondria.
Experimental diabetes mellitus was evoked by an injection of streptozotocin in male Wistar
rats. After 7 days half the rats were administered PAMAM 64 i.p. daily. After 8 weeks the
concentration of glucose, the end-products of advanced glycation and the end-products
of advanced protein oxidation in plasma and glycated haemoglobin in blood were determined.
Liver tissue was used for mitochondria isolation and parameters of oxidative
phosphorylation were measured using volt-amper method with a Clark oxygen electrode.
Our results, for the first time in vivo experiment, show that PAMAM G4, regardless of
its known cytotoxicity, significantly reduced hyperglycaemia and all the measured longterm
markers of hyperglycaemia in diabetic rats. This positive effect is not sufficient to
restore the impaired mitochondrial function in experimental diabetes.
Acknowledgement: Supported by grant VEGA 1/0328/10.
176 XXII. Biochemistry Congress, Martin