XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta
XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta
XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta
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Posters<br />
53.<br />
DISTRIBUTION AND BIOCHEMICAL CHaraCTERIZATION OF CD52-LIKE<br />
MOLECULE IN BULL EPIDIDYMIS<br />
Katarína Michalková, Michal Simon, Jana Antalíková and Ľubica Horovská<br />
Institute of Animal Biochemistry and Genetics SAS Ivanka pri Dunaji<br />
The aim of this study was to analyze the distribution and biochemical properties of the<br />
antigen in bull genital tract using monoclonal antibody IVA-543 produced in IABG SAS.<br />
CD52 is a GPI linked protein with a molecular weight of about 25 to 29 kDa, it is expressed<br />
on all lymphocytes, and in male genital tract. Except humans, CD52 has been described<br />
in mice, rats, chimps and dogs. Characteristic common to all species is very similar nature<br />
of expression in cells of the epididymal epithelium, the protein in the genital tract<br />
is produced post-testicular and constitutes the main surface glycopeptide of the sperm<br />
from the corpus and cauda epididymis. Our results revealed that CD52-like molecule in<br />
the reproduction system of bull is produced by epididymal epithelial cells and secreted<br />
into the lumen. Immunohistochemical analysis confirmed that the antigen is synthesized<br />
in the epididymis downstream, in the largest quantities it can be detected in the cauda<br />
epididymal tissue. The similar results we obtained by western blot analysis of the cauda<br />
epididymal fluid. FACS analysis and the indirect immunofluorescence of sperm from of<br />
epididymis confirmed these results, in the proximal part of epididymis CD52-like molecule<br />
is present on 4.73 % of sperm and in the distal part it is present on up to 99.45 % of the<br />
sperm. Bovine antigen expression in the distal part of the epididymis points out that<br />
the bull sperm acquire CD52-like molecule on their surface during their transit through<br />
the epididymis, especially in the distal part of this organ. Western blot analysis showed<br />
that the molecular weight of this bull epididymal antigen derived from tissue, fluid or<br />
from sperm is ranging from 22 to 25 kDa.<br />
Acknowledgments: This work was supported by grants VEGA-2/6023/27 and<br />
APVV-VVCE-0064-07.<br />
<strong>XXII</strong>. Biochemistry Congress, Martin<br />
173
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