XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Posters
38.
ALTERED CALCIUM SIGNALING IN PERIPHEraL BLOOD MONONUCLEar
CELLS OF CHRONIC KIDNEY DISEASE PATIENTS
Ingrid Lajdová 1 , Viera Spustová 1 , Adrián Okša 1 and Dušan Chorvát Jr. 2
1
Slovak Medical University, Department of Clinical and Experimental Pharmacotherapy,
2
International Laser Centre, Department of Biophotonics, Bratislava
A rise in the intracellular free calcium concentration ([Ca 2+ ] i
) is a key signal in the initialization
of wide range of cellular events. The elevation of [Ca 2+ ] i
have been found in association
with chronic kidney disease (CKD). The aim of this study was to investigate the [Ca 2+ ] i
,
intracellular calcium reserves, capacitative calcium entry and function of purinergic P2X 7
receptors in peripheral blood mononuclear cells (PBMCs) of early-stage CKD patients.
The study involved 22 healthy volunteers and 22 patients in CKD stage 2-3. The peripheral
blood mononuclear cells (PBMCs) were separated by the Ficoll gradient centrifugation,
and free intracellular calcium was measured using the Fluo-3 AM fluorimetry. The P2X 7
pore function was evaluated by using a fluorescent dye ethidium bromide.
Our results demonstrate that 1) [Ca 2+ ] i
, intracellular calcium stores and the capacitative
calcium entry were significantly increased already in early stages of CKD; 2) purinergic
P2X 7
receptors participate in intracellular calcium homeostasis regulation in CKD.
Acknowledgements: Supported by grants No. APVT-21-033002 and VG SZU 19-90-07
156 XXII. Biochemistry Congress, Martin