XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Posters
35.
GENEraTION aND CHaraCTErIZaTION MONOCLONal aNTIBODIES
agaINST ENDOSIaLIN, THE POTENTIal marKEr of TUMOr
aNGIOGENESIS
Soňa Kontseková, Anna Repič, Monika Baráthová,
Katarína Polčicová and Jaromír Pastorek
Institute of Virology, SAS, Dúbravská cesta 9, 84505 Bratislava
Endosialin, also known as TEM1, or CD248, was first discovered with the monoclonal
antibody (mAb) Fb5 as an experimental approach to identify new targets for anti-cancer
strategies. It was described as a transmembrane glycoprotein selectively expressed on
tumor endothelium, but its expression is barely detectable in normal human tissue.
Recent experiments have challenged the endothelial expression of endosialin and suggested
an expression by activated fibroblasts and pericytes. Subsequent studies have
also confirmed that endosialin is upregulated in blood vessels in wide range of tumors
and its expression is restricted to capillaries, which means that endosialin is probably
involved in vascular reorganisation. It has been functionally implicated in angiogenesis,
a process characterized by vascular branching and sprouting, which is necessary for tumor
expansion and progression. In this work, we generated monoclonal antibodies against
endosialin, characterized their isotypes and binding epitopes. We also analysed the
activity of antibodies in a set of imunological assays and characterized the most stabile
antibody VIII-16 with potential usage in next studies of endosialin function.
Acknowledgements: This work was supported by VEGA 2/0210/09 and TRANSMED.
XXII. Biochemistry Congress, Martin
153