XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Posters
26.
THE ASSOCIATION BETWEEN EGF 61 G/A POLYMORPHISM AND
COLORECTAL CANCER DEVELOPMENT
Silvia Mahmood, Tatiana Matáková, Lucia Letková, Monika Kmeťová Sivoňová,
Jozef Hatok and Dušan Dobrota
Department of Medical Biochemistry, Jessenius Faculty of Medicine,
Comenius University, Martin, Slovakia
The epidermal growth factor (EGF) gene has been demonstrated to participate in the
pathogenesis or maintenance of several human cancers of epithelial origin. The purpose
of the present case-control study was to investigate the association of the single-nucleotide
polymorphism of EGF + 61 G/A with the susceptibility to colorectal cancer (CRC)
in a population in the north of Slovakia. We have analyzed the genotype distribution of
this polymorphism in 120 patients (65 [54,2%] men and 55 [45,8%] women, mean age
64,2 years) and 110 healthy subjects, using the PCR-RFLP technique. Differences in allele
and genotype frequencies were evaluated by the Chi-square (c 2 ) and Fisher exact tests.
Our data suggests that EGF +61 G/A polymorphism may be used as a genetic susceptibility
marker for CRC. With the aim to inhibit cancer growth and to reduce the risk of
metastasis we adapt an avascular tumour growth model to simulate the effects of drug
application on multicell spheroid (MCS) [1, 2].
Acknowledgement: This work was supported by project “Creating a new diagnostic algorithm
for selected cancer diseases” co-financed from EC sources and European Regional
Development Fund.
References
(1) Mahmood, M., Mahmood, S. Dobrota, D. (2010): A numerical algorithm for avascular
tumor growth model. Math. Comp. Sim., Vol. 80 (6): 1269-1277.
(2) Ward JP, King JR (2003): Mathematical modelling of drug transport in tumour MCS
and monolayer cultures. Math. Biosci., 181(2):177-207.
XXII. Biochemistry Congress, Martin
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