XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta
XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta
XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta
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Posters<br />
24.<br />
DNA POLYMOrPHISMS of SELECTED rEPair GENES aND rISK of LUNG<br />
caNCEr<br />
Lucia Letková 1 , Tatiana Matáková 1 , Erika Halašová 2 ,<br />
Anna Drgová 1 and Dušan Dobrota 1<br />
1<br />
Department of Medical Biochemistry; 2 Department of Medical Biology,<br />
Comenius University, Jessenius Faculty of Medicine, Martin, Slovakia<br />
The presence of polymorphisms of repair genes contributes to individual susceptibility<br />
to the development of lung cancer. We investigated the association between polymorphisms<br />
in DNA repair genes hOGG1 (Ser326Cys) and XRCC1 (Arg399Gln), which play an<br />
important role in DNA base excision repair. In this case-control study, we recruited 309<br />
lung cancer cases and 339 healthy controls. Genomic DNA was extracted from peripheral<br />
blood by phenol/chloroform extraction. The genotypes of hOGG1 and XRCC1genes were<br />
determined by PCR-RFLP method. The differences in the distribution of genotypes and<br />
alleles between cases and controls were analysed using x 2 -test. The odds ratio (OR) and<br />
95% confidence intervals (95% Cl) were adjusted for the risk of developing lung cancer,<br />
alone polymorphisms or their combinations. The presence of polymorphisms of genes<br />
hOGG1 and XRCC1 in women represents elevated risk of lung cancer compared to men.<br />
The frequency of the genotype XRCC1 Gln/Gln (OR=2,32; P=0,19) represented 2-fold<br />
higher risk of lung cancer compared with genotype Arg/Arg in women. Genotypes Arg/<br />
Arg+Ser/Cys (OR= 2,45; P=0,19), Arg/Gln+Ser/Ser (OR=2,3; P=0,08), Arg/Gln+Cys/Cys<br />
(OR=2,45; P=0,76) had a 2-fold increase risk of lung cancer compared with Arg/Arg+Ser/<br />
Ser genotype. The frequency of the Gln/Gln+Ser/Ser (OR=7,36; P=0,012) genotypes was<br />
statistically significantly higher compared with Arg/Arg+Ser/Ser genotype. In summary,<br />
variants alleles Cys326 and Gln399 are associated with reduce enzyme activity of genes<br />
and DNA repair capacity. The presence of these alleles leads to increase susceptibility<br />
at risk of developing lung cancer.<br />
<strong>XXII</strong>. Biochemistry Congress, Martin<br />
141
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