XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Posters
14.
CONFORMATION TraNSITIONS OF CYTOCHROME C IN DEEP EUTECTIC
SOLVENTS
Jozef Parnica 1 , Lukáš Kandráč 1 and Marián Antalík 1, 2
1
Department of Biochemistry, Faculty of Science, P.J. Safarik University, Kosice,
Slovak Republic, 2 Institute of Experimental Physics, SAS, Kosice, Slovak Republic
Original deep eutectic mixtures (DES) were formed by mixing two components together in
different molar ratios that had a melting point much lower than any of the constituents,
until homogenous, colorless liquid was formed. Deep eutectic solvents were also included
in the advanced ionic liquids. They have recently attracted much attention as “green”
alternatives to conventional organic solvents in various fields including biochemistry and
separation processes due to their unique properties such as high thermal stability, negligible
vapor pressure and non-flammability. In our work, we applied DES’s as alternative
solvents to determinate the stability and conformation transitions of a model protein,
ferricytochrome c. We used urea, malonic acid, and sorbitol as hydrogen bond donors
by UV-Vis spectroscopy and circular dichroism spectroscopy in mixtures of substituted
quaternary ammonium salts e.g. hydroxyethyltrimethyl-ammonium (choline) chloride.
We have found DES’s as suitable solvents for characterization new types of conformation
states of ferricytochrome c that demonstrate their advantages and unique properties,
and suggest opportunities for new applications.
Acknowledgement: This work was supported within the projects Nos., 26220120001,
26220220005, 2622022033 in frame of SF EU, Centre of Excellence of SAS Nanofluid and
VEGA 0056, 0038 and 0079.
XXII. Biochemistry Congress, Martin
129