XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Posters
6.
ANATOMICAL DISTRIBUTION OF DYING CELLS WITHIN ADULT raTS
ROSTraL MIGraTORY STREAM
Marcela Martončíková, Kamila Lievajová, Juraj Blaško,
Judita Orendáčová and Enikő Račeková
Institute of Neurobiology, Slovak Academy of Sciences, Košice, Slovak Republic
The rostral migratory stream (RMS) is a pathway along which newborn cells originated
from the subventricular zone migrate toward the olfactory bulb where they differentiate
into interneurons. This migratory pathway consists of three parts in caudo-rostral
direction: the vertical arm, the elbow and the horizontal arm. The precursor cells are
able to proliferate during migration in the RMS. It has been reported that these cells may
also undergo apoptotic cell death. Dying cells has been observed along entire extent
of the RMS. The number and distribution of dying cells in the RMS varies according to
the methodology used. Commonly applied methodology for identification of apoptotic
cells is a terminal dUTP nick-end-labelling (TUNEL). Another marker for detection of dying
cells is a fluorochrome, Flouro Jade-C (FJ-C), which stains all degenerating neurons,
regardless of mechanism of cell death. Studies dealing with the cell death in the RMS
assess the total number of positive cells mainly in whole extent of the RMS. In this
study we focused on the distribution of FJ-C positive cells in individual anatomical parts
of the RMS. We found that the number of dying cell is the highest in the caudal part
of the RMS – in the vertical arm. The lowest number of dying cells was observed in the
middle part of the RMS –in the elbow. In the rostral part of the RMS- in the horizontal
arm the number of these cells was higher than in the elbow. Differences in the number
of dying cells in the individual parts of the RMS are probably associated with different
developmental origin of these parts.
Acknowledgments: Supported by VEGA grants: 2/0147/09; 2/058/08.
120 XXII. Biochemistry Congress, Martin