XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Posters
3.
SELECTED GENE POLYMORPHISMS IN ISCHEMIC STrOKE aND
DEPRESSED HUMan paTIENTS frOM CENTraL SLOvaKIA
1
Andrea Evinová, 1 Eva Babušíková, 2 Pavol Adamík, 2 Igor Ondrejka, 3 Egon Kurča,
3
Milan Grófik and 1 Ján Lehotský
Comenius University, Jessenius Faculty of Medicine, Department of Medical
Biochemistry 1 , Psychiatry Clinic 2 , Neurological Clinic 3 , Martin, Slovakia
The neurobiological basis of ischemic stroke and postischemic depression is not yet clarified.
We have focused on several genes which could be connected with the pathogenesis
of ischemic stroke and depression. Angiotensin converting enzyme (ACE), as a part of
the renin-angiotensin system, is important factor in blood pressure regulation. Incidence
of D allele is associated with higher protein level and its activity. Serotonin-transporterlinked
promoter (HTTLPR) is a d egenerate polymorphic region in the gene that codes the
serotonin transporter. Its polymorphism is connected with neuropsychiatric disorders.
Likewise, S allele is associated with the functional reduction as compared to L allele.
Glutathione-S-tranferases (GST) detoxify a broad range of xenobiotics and carcinogens.
The most studied polymorphisms are in families of GSTM1, GSTT1 and GSTP1. In our
study we tested the gene polymorphisms in three groups of patients, with: i) ischemic
stroke ii) depression, and iii) healthy controls. In the DD polymorphism of ACE gene, the
depressed pacients exhibit higher frequency of DD genotypes in comparison to controls.
Stroke patients exhibit only non-significantly lower frequency in comparison to controls.
So far, we did not observe any significant differences in allelic frequency for HTTLPR
gene neither for depressed nor ischemic groups in comparison to controls. Our results
suggest that ACE DD genotype is increased in depressed pacients. Analysis of selected
polymorphisms of GSTM1 and GSTT1 genes show higher frequency of GSTM1 null and
GSTT1 wild genotypes.
This work was supported by the VEGA 49/09 and MZ-2007/55-UK-16
XXII. Biochemistry Congress, Martin
117