Development of a Novel Mass Spectrometric ... - Jacobs University

Experimental
2 Experimental
2.1 Chemicals and Model Standards
a) Standard low mass n-alkanes such as Octane, nonane, decane, undecane,
dodecane, tridecane, tetradecane, pentadecane and hexadecane were purchased
from Sigma Aldrich (Bremen, Germany). Higher mass n-alkanes like octadecane,
eicosane, henicosane, docosane, tricosane, tetracosane, pentacosane, hexacosane,
octacosane, dotricontane, hexatriacontane, tetracontane, dotetracontane,
tetratetracontane, octatetracontane, pentacontane, tetrapentacontane and
hexacontane all of analytical grade, were purchased from Sigma Aldrich.
b) Different model mixtures of n-alkanes were also purchased from the same
company.
i) C7-C40 Saturated Alkanes (1 mg/ml in hexane)
ii) C8-C40 Alkanes Calibration Standard (500 µg/ml in dichloromethane)
iii) C10, C20-C40 Alkane Standard Mixture (50 mg/l in n-heptane)
iv) C21-C40 Alkane Standard Solution (40 mh/l in toluene)
v) C12-C60 Quantitative Linearity Standard (0.01 % (w/w) in cyclohexane)
vi) n-Paraffin Mix C18, C20, C22, C24 Analytical Standard (2 % (w/w) in octane)
c) Other deuterated standards such as deuterated tetracosane [(D-24), (C 24 D 50 )] and
deuterated dotriacontane [(D-32), (C 32 D 66 )]
d) Other individual standards were purchased from Chiron Company (Chiron.no).
These standards were 2,6,10,14-tetramethyl nonadecane, 2,6,10,14,18-pentamethyl
heneicosane, n-tetradecyl cyclohexane, n-octadecyl cyclohexane, n-octyl benzene,
n-nonyl benzene, n-tetradecyl benzene, and n-octadecyl benzene, each in 1 mg/ml
isooctane solution. 5-α-Cholestane, squalane, squalene, phytane, and 1-phenyl
decane all of analytical grade were also purchased from Sigma Aldrich.
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