07.03.2014 Views

CombiFlash Torrent® User Manual - Isco

CombiFlash Torrent® User Manual - Isco

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Section 4 <strong>User</strong> Help Reference<br />

• Insert Point — This command or button will insert a<br />

row below the selected point.<br />

• Delete Point — This command will delete the selected<br />

row. Note that you cannot delete the initial point.<br />

PeakTrak automatically updates the Run Length setting or<br />

scales the other points when you change the number of points<br />

and their duration on the curve.<br />

To close the Gradient Table, click the Edit Gradient button.<br />

See Also: DEFINING A GRADIENT [4.4.5]<br />

4.3.2.5 Peak Collection<br />

Use the Peak Collection buttons to set the collection mode.<br />

• All — Click this button to collect all fluids during a run.<br />

• Peaks — Click this button to collect only eluted peaks<br />

during a run.<br />

• None — Click this button to divert all fluids to the<br />

waste port during a run.<br />

• Tube Volume — You can also specify the Tube Volume<br />

for collected fluids. This volume can be the default<br />

maximum volume (Max option) as entered in the<br />

CONFIGURATION [4.3.10] window, or a method-specific<br />

volume less than the capacity defined by the<br />

CONFIGURATION [4.3.10] window. When using a Foxy<br />

fraction collector or the Fractionation Valve, note that<br />

the actual fraction size may be less if a newly detected<br />

peak causes a tube change, or if you click the Next Tube<br />

button.<br />

• Peak — Type or select the desired volume to be<br />

collected in each tube when the module detects a<br />

peak.<br />

• Non-peak — Type or select the desired volume to be<br />

collected in each tube when peaks are not detected.<br />

This setting allows you to conserve tubes without<br />

diverting non-peak elute to waste. This setting is<br />

ignored when the peak collection mode is set to Peaks<br />

or None.<br />

4-19

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