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CombiFlash Torrent® User Manual - Isco

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<strong>CombiFlash</strong> Torrent® <strong>User</strong> <strong>Manual</strong><br />

once for each gradient point that must be added to the<br />

plot area.<br />

• Delete —Click this button to enable the gradient point<br />

delete mode. When this mode is active, PeakTrak will<br />

delete the point nearest the next click on the gradient<br />

curve. Click the Delete button once for each point that<br />

must be deleted from the plot area.<br />

• Run Length — Type or select the length of the run.<br />

Note that this will change the scale of the X-axis on the<br />

gradient. Points that define the gradient will be<br />

automatically scaled to fit the new run length. The run<br />

length can be expressed in minutes or COLUMN VOLUMES<br />

[ON-LINE] (CV). You can configure PeakTrak’s default Run<br />

Length units by changing the CONFIGURATION [4.3.10]<br />

settings.<br />

• Solvent A — Select the desired solvent from the list.<br />

The available solvents are those defined by the<br />

CONFIGURATION [4.3.10] settings.<br />

• Solvent B — Select the desired solvent from the list.<br />

You can program mid-run solvent B changes by clicking<br />

the Edit Gradient button to open the GRADIENT TABLE.<br />

• Edit Gradient — Although the gradient may be edited<br />

directly within the plot area, you can also edit the<br />

gradient in tabular form. Click this button to open the<br />

GRADIENT TABLE.<br />

For complete instructions on defining gradient curves, see<br />

DEFINING A GRADIENT [4.4.5].<br />

Gradient Table The gradient table depicts the points that<br />

define the gradient curve in a tabular format. From this table<br />

you can change the duration and %B concentration of any<br />

point on the curve. To do so, select new values for the gradient<br />

points. Changes that you make to the table will be reflected in<br />

the gradient plot area.<br />

You can also change the number of points on the curve by<br />

inserting or deleting rows in the table. To change the number<br />

of points, first highlight a table cell or row. Then click the<br />

appropriate Gradient Button for the action you desire.<br />

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