CombiFlash Torrent® User Manual - Isco
CombiFlash Torrent® User Manual - Isco
CombiFlash Torrent® User Manual - Isco
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Section 4 <strong>User</strong> Help Reference<br />
Time Windows can limit the fraction collection to specific<br />
time durations of the run. To use time windows, first<br />
enter a start time and an end time. Note that all times<br />
are relative to start of the run, just following the sample<br />
injection. Then choose a peak collection mode. If you<br />
select Peaks the module will collect fluid only when the<br />
time window is active and a peak is present. If you select<br />
All the module will collect all fluid during the time<br />
window regardless of the peak state. Define up to three<br />
time windows.<br />
• Air Purge — Type or select the duration of a post-run<br />
air purge, in minutes. An air purge clears the solvent<br />
from the column and the fluid path, forcing any liquid<br />
out the waste port. If you are disposing the column after<br />
each run, a sufficient post-run air purge ensures that it<br />
will be empty. To disable the air purge, enter a value of 0<br />
(zero). A column can also be manually air purged. See<br />
MANUAL CONTROL [4.3.9].<br />
4.3.2.3 Run Notes<br />
Use this text entry box to enter comments or notes for the run.<br />
These comments will be saved with the run and will appear in<br />
TXT and PDF reports.<br />
4.3.2.4 Gradient Plot Area<br />
The gradient plot area depicts the current gradient profile<br />
that will be used for the run. The X-axis of the plot displays<br />
the run time in minutes or COLUMN VOLUMES [ON-LINE]. The<br />
X-axis scale can be adjusted by typing or selecting the Run<br />
Length. The left Y-axis displays absorbance units and the<br />
right Y-axis indicates the percentage of solvent B.<br />
The gradient plot area also provides a convenient method to<br />
edit the gradient curve. You can click and drag any of the<br />
points to change the shape of the curve, or use the buttons at<br />
the bottom of the plot area to insert and delete points. The<br />
following controls appear in the Gradient Plot Area:<br />
• Insert — Click this button to enable the gradient point<br />
insert mode. When this mode is active, click the gradient<br />
curve to add a single point. You can then drag the new<br />
point to any desired position. Click the insert button<br />
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