CombiFlash Torrent® User Manual - Isco

CombiFlash Torrent® User Manual
PeakTrak’s default Run Length units by changing the
CONFIGURATION [4.3.10] settings.
4.3.2.2 Run Settings
• RediSep Column — The RediSep column media and
size are detected by PeakTrak and reported here. The
column should match the one that will be used for the
run. You may override the detected column size by
selecting a different size. See also: REDISEP RF COLUMN
SELECTION GUIDE [ON-LINE] and ABOUT REDISEP RF
COLUMN DETECTION [ON-LINE].
• Sample Name — The sample name is a text entry box
in which you can label the run. Type a unique descriptor.
PeakTrak saves the run information under this name.
Therefore, PeakTrak cannot accept any characters that
are reserved by the operating system (* ? / \, etc.). If you
do not type a Sample Name, PeakTrak will generate a
date/time stamp for the name when you click the Play
button to begin the run.
• Flow Rate — Type or select the desired flow rate for the
run. When using the Isco default column methods, the
module sets the flow rate to the optimum flow rate of the
column.
• Equilibration Volume — Type or select the volume of
solvent that is pumped through the column and out the
waste port before the sample is introduced. Data is not
collected while this volume is being pumped. Three or
more column volumes are recommended for most
applications.
• Initial Waste & Time Windows — Click this button to
open a window from which you can view and modify
these settings. For the Initial Waste, type or select the
volume of eluate that is allowed to pass through the
column to the waste port after the sample is injected.
After this volume has been diverted, the module is ready
to collect peak or all fluids in the collection tubes. Use
this feature to clear the internal lines of any elute left
from a previous run and to conserve collection tubes by
diverting all fluids until the first peak is expected.
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