CombiFlash Torrent® User Manual - Isco

Section 4 User Help Reference
the left side of the window changes to show a collection point
map or the method parameters in a PEAK COLLECTION DATA
[4.3.1.5] pane.
4.3.1.1 Gradient Plot
The gradient plot area depicts the current gradient that will
be used for the run. The X-axis of the plot displays the run
time in minutes or COLUMN VOLUMES [ON-LINE]. The X-axis scale
can be adjusted by typing or selecting the Run Length. The
left Y-axis displays absorbance units and the right Y-axis
indicates the percentage of solvent B. By default, PeakTrak
automatically scales the left Y-axis to best display the
absorbance trace. PeakTrak also sets the right Y-axis to 100%.
You can override the Y-axes scales by clicking the Format
Graph button and setting the desired values.
The Cartridge Bypass button may be used during a run to
relieve backpressure caused by impurities precipitating in the
solid load cartridge. Use this feature with care to avoid leaving
desired compound on the cartridge. Read more about this
feature in BYPASSING THE SOLID LOAD CARTRIDGE [4.4.8]
If the external detector option is enabled, the plot area will
include a secondary left Y-axis to display the analog input
trace.
The gradient plot area also provides a convenient method to
edit the gradient curve. You can click and drag any of the
points to change the shape of the curve and use the buttons at
the bottom of the plot area to insert and delete points. For
complete instructions on defining gradient curves, see
DEFINING A GRADIENT [4.4.5].
4.3.1.2 Run Settings
• RediSep — PeakTrak reports the detected RediSep
column media and size. The column should match the
one that will be used for the run. You may override the
detected column size by selecting a different size or
media. See also: REDISEP RF COLUMN SELECTION GUIDE
[ON-LINE] and ABOUT REDISEP RF COLUMN DETECTION
[ON-LINE].
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