CombiFlash Torrent® User Manual - Isco
CombiFlash Torrent® User Manual - Isco
CombiFlash Torrent® User Manual - Isco
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
Section 3 Operation<br />
Note<br />
When you click the Next Tube button, the system immediately<br />
marks the tube advance on the chromatogram. However, the<br />
system delays the movement of the drop former over the tubes.<br />
This delay accounts for the volume held in the tubing between the<br />
detector and the drop former, sometimes called the “delay<br />
volume.” It is important to remember that the chromatogram<br />
displays activity in real time while the fraction collector activity lags<br />
behind. The duration of this delay will vary with the flow rate.<br />
• Rewind — This button is active when the system has<br />
completed the run, or if the run was stopped by clicking<br />
the Stop button. The Rewind button returns you to the<br />
Main window with the current method.<br />
If you have stopped the run before its programmed run<br />
length has elapsed, clicking the Rewind button changes the<br />
current method to the new, shorter run length. This modified<br />
method is ready for the next run, or can be saved for<br />
future runs.<br />
• Fast Forward — Click this button to jump to the next<br />
step of a run.<br />
Once you have started a run, the system performs several<br />
steps. The first step is to deliver solvents using the programmed<br />
gradient for the entire run length. When this step<br />
is complete, the system will purge the column with air and<br />
clean the injection valve. Clicking the Fast Forward button<br />
will cause the system to skip any remaining time in the<br />
current step and advance to the beginning of the next step.<br />
Unlike the Rewind button, clicking the Fast Forward button<br />
to complete the run will not modify the current<br />
method’s run length setting.<br />
Note<br />
Solvent may be under pressure. Do not remove a column or solid<br />
load cartridge until the pressure gauge on the touch screen to<br />
return to 0 psi.<br />
3-17