CombiFlash Torrent® User Manual - Isco
CombiFlash Torrent® User Manual - Isco
CombiFlash Torrent® User Manual - Isco
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
<strong>CombiFlash</strong> Torrent® <strong>User</strong> <strong>Manual</strong><br />
CAUTION<br />
When the Stop button is pressed, solvent remains in the tubing<br />
from the injection valve to the detector’s flow cell. Follow the steps<br />
listed below, according to your instrument’s configuration.<br />
• Solid Sample Cartridge and air purge is enabled:<br />
If you suspend the run with the Stop button, do not remove<br />
the column and/or solid sample cartridge without first<br />
pressing the Fast Forward button to purge solvents from all<br />
lines. If you stop the run because your peak of interest has<br />
been collected, protect the next user from being sprayed by<br />
solvents left in the lines by pressing Fast Forward so the<br />
instrument purges itself and is safe for its next use.<br />
• Solid Sample Cartridge and air purge is NOT enabled,<br />
i.e. purge time set to zero in Method Editor:<br />
If you suspend the run with the Stop button, remove the<br />
column and install the Prime Tube in place of the column.<br />
<strong>Manual</strong>ly purge the Sample Cartridge by pressing Tools -><br />
<strong>Manual</strong> Control -> Air Purge Cartridge. Repeat this<br />
procedure for Air Purge Column.<br />
• Liquid sample is loaded onto column and air purge is<br />
enabled:<br />
If you suspend the run with the Stop button, remove the<br />
column and install the Prime Tube in its place. Press Fast<br />
Forward to purge all liquid lines.<br />
• Liquid sample loaded onto column with air purge NOT<br />
enabled:<br />
If you suspend the run with the Stop button, remove the<br />
column and install the Prime Tube in its place. <strong>Manual</strong>ly<br />
purge the liquid lines by pressing Tools -> <strong>Manual</strong> Control<br />
-> Air Purge Column.<br />
• Next Tube — This button advances the fraction<br />
collector/fractionation valve to the next tube position.<br />
This allows you to conveniently collect eluate of interest<br />
in a new tube.<br />
3-16