CombiFlash Torrent® User Manual - Isco

Section 3 Operation
3.5 During the Run
You can monitor the progress of the run on the PeakTrak main
window. If desired, you can modify gradient parameters
during the run. (See the Real-time Gradient Editing on-line
help topic.)
As the run progresses, the absorbance trace is drawn on the
Gradient Plot area. The separation or purification continues
until the end of the run defined by the run length setting.
During the run, data is automatically saved every few seconds.
PeakTrak automatically saves all run history on the system’s
internal hard drive in its native .RUN format. You can
manually save a Run file as Text or PDF while stopped or any
later time as long as the .RUN file has not been deleted.
Selected files can be manually saved to a connected Flash
drive. To automatically save a Text or PDF file after each run,
configure the Network File Save feature (see Section 5.1.3).
3.5.1 Run Control Buttons
• Play — The Play button starts the run. During the run,
other run control buttons are active.
• Pause — The Pause button holds the %B at the current
value while the system continues to operate (sometimes
called an isocratic hold). Note that pausing the run
extends the run length. While in the Paused state, you
can resume the run by clicking the Play button, or stop
the run by clicking the Stop button. If you resume the
run, the system continues with the gradient curve at the
%B when the system was paused.
• Stop — The Stop button suspends the entire run.
Unlike the Paused state, the pump, peak detection, and
fraction collection will not operate. While stopped, you
can end the run by clicking the Rewind button, resume
the run by clicking the Play button, or skip the rest of
the run sequence by clicking the Fast Forward button.
3-15