CombiFlash Torrent® User Manual - Isco
CombiFlash Torrent® User Manual - Isco
CombiFlash Torrent® User Manual - Isco
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Section 3 Operation<br />
3.5 During the Run<br />
You can monitor the progress of the run on the PeakTrak main<br />
window. If desired, you can modify gradient parameters<br />
during the run. (See the Real-time Gradient Editing on-line<br />
help topic.)<br />
As the run progresses, the absorbance trace is drawn on the<br />
Gradient Plot area. The separation or purification continues<br />
until the end of the run defined by the run length setting.<br />
During the run, data is automatically saved every few seconds.<br />
PeakTrak automatically saves all run history on the system’s<br />
internal hard drive in its native .RUN format. You can<br />
manually save a Run file as Text or PDF while stopped or any<br />
later time as long as the .RUN file has not been deleted.<br />
Selected files can be manually saved to a connected Flash<br />
drive. To automatically save a Text or PDF file after each run,<br />
configure the Network File Save feature (see Section 5.1.3).<br />
3.5.1 Run Control Buttons<br />
• Play — The Play button starts the run. During the run,<br />
other run control buttons are active.<br />
• Pause — The Pause button holds the %B at the current<br />
value while the system continues to operate (sometimes<br />
called an isocratic hold). Note that pausing the run<br />
extends the run length. While in the Paused state, you<br />
can resume the run by clicking the Play button, or stop<br />
the run by clicking the Stop button. If you resume the<br />
run, the system continues with the gradient curve at the<br />
%B when the system was paused.<br />
• Stop — The Stop button suspends the entire run.<br />
Unlike the Paused state, the pump, peak detection, and<br />
fraction collection will not operate. While stopped, you<br />
can end the run by clicking the Rewind button, resume<br />
the run by clicking the Play button, or skip the rest of<br />
the run sequence by clicking the Fast Forward button.<br />
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