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CombiFlash Torrent® User Manual - Isco

CombiFlash Torrent® User Manual - Isco

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<strong>CombiFlash</strong> Torrent® <strong>User</strong> <strong>Manual</strong><br />

Figure 3-7 Sample Load Pump options<br />

2. Prepare the Sample Load pump to receive the sample.<br />

If the sample mixture will be transferred to the pump,<br />

insert the cartridge onto the pump inlet (Figure 3-7, Left).<br />

Then, fill the cartridge with the mixture.<br />

If you will transfer the sample mixture directly from a container,<br />

attach the tubing to the pump inlet (Figure 3-7,<br />

Right). Place the weighted end into the mixture container.<br />

3. Place the On/Off (I/O) switch in the On position. Adjust the<br />

pump speed using the control knob.<br />

4. When the desired sample volume has been loaded, place<br />

the On/Off (I/O) switch in the Off position.<br />

Note<br />

After use, always pump clean solvent through the Sample Load<br />

Pump to remove sample residue.<br />

Note<br />

Some of the sample mixture will remain in the tubing before the<br />

automated injection valve. In most applications, pump a chase<br />

solution to force the sample mixture through the injection valve. To<br />

do so, repeat steps 2 through 4 using a suitable chase solvent.<br />

3-14

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