CombiFlash Torrent® User Manual - Isco

Section 3 Operation
3. Open the valve.
4. Inject the liquid sample mixture.
5. Close the valve and remove the syringe.
Note
Chase solvent clears the Liquid Injection Adapter tubing and
forces the liquid compound mixture onto the column. Without the
chase solvent, some of the sample will remain in the tubing before
the automated injection valve and will not be separated. This small
amount of sample may also cross-contaminate the next purification
run. The chase solvent should be strong enough to prevent
the sample from precipitating (or crashing), but not so strong as to
adversely affect the separation of compounds. Often, the initial liquid
phase solvent is a practical chase solvent.
6. Inject a chase solvent:
a. Connect a syringe loaded with a chase solvent to the
Liquid Injection Adapter’s luer fitting.
b. Open the valve by turning the handle so it is in line with
the tubing.
c. Inject at least 10 mL of the chase solvent.
d. Close the valve by turning the handle so that it is
perpendicular to the tubing. Remove the syringe.
7. Click the OK button to continue the run.
Note
Chase solvent will remain in the line up to the automated injection
valve. Keep in mind that this solvent may affect the next injection.
3.4.2 Sample Load Pump
1. Connect the outlet of the Sample Load pump to the
Torrent’s Sample Injection Port.
Note
To bypass the injection valve, the sample may also be pumped
onto to the column at the upper column mount. If this method is
preferred, use the Direct Injection Adapter on the upper column
mount to ease tubing changes. (See section 3.1.3.)
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