CombiFlash Torrent® User Manual - Isco

CombiFlash Torrent® User Manual
• If you selected Solid (Pause), the system
equilibrates the column with the starting %B solvent
mixture and then waits while you prepare the solid
sample load cartridge. When prompted, place the
cartridge on the system and click OK to continue.
• If you selected Solid, the system equilibrates the
column and immediately starts the separation. The
prepared solid load cartridge should be in place
before clicking the OK button.
• If you selected None (on Column), the system skips
the equilibration and immediately starts the
separation. The sample must be on the column before
clicking the OK button.
• If you selected Liquid, the system equilibrates the
column and then waits. When instructed, load the
liquid sample using the syringe injection (3.4.1) or the
Sample Load Pump (3.4.2) options.
3.4.1 Syringe Injection
Refer to Figure 3-6 for the following steps.
1. Connect the Liquid Injection Adapter from the accessory
kit to the Sample Injection Port.
2. Place the syringe on top of the Liquid Injection Adapter
valve.
Closed: handle
perpendicular
to tubing
Open: handle
in-line with tubing
Figure 3-6 Liquid Injection Adapter
3-12