CombiFlash Torrent® User Manual - Isco

Section 3 Operation
cartridge. After you have placed the cartridge on the
system, click OK to continue with the run.
• Solid – Select this option if you already have
prepared the sample and placed it into a solid sample
load cartridge. The system will proceed automatically
until the end of the programmed run.
• Liquid – Select this option if you have prepared a
liquid sample and plan to manually inject it or pump
into the injection port after column equilibration.
• None (on column) – Select this option if you have
preloaded the sample on the column. The system will
skip column equilibration so that the sample will not
be flushed from the column before the run.
5. Review or update the Start Rack and Start Tube settings.
6. Review the Minimum Run Requirements. The system will
report the estimated solvent volumes, expected waste,
collection tube usage, etc. You can use this information to
verify that there will be enough solvent to complete the
run, the waste volume does not exceed the collection
container’s capacity, and whether or not more collection
tubes will be required during the run.
Note
The Torrent estimates the solvent volumes by monitoring the
solvent level in the container and the known usage rate. The
systems continue to refine this estimate during operation.
Note
If the waste level sensing tube is inserted correctly into the waste
container, the Torrent will automatically suspend operation before
an overflow condition might exist. To prevent the run from being
suspended before completing the run, ensure that the container
will hold the expected waste volume.
7. Click OK and the run begins. The system responds
according to the sample Loading Type you selected in
step 4.
3-11