CombiFlash Torrent® User Manual - Isco

CombiFlash Torrent® User Manual
Note
After loading a RediSep Rf column, the system will use RFID
technology to automatically detect the media type and column
size. PeakTrak displays the detected column size on the Main and
Method Editor windows. If the system does not detect the column,
manually select the column media and size.
3.4 Start a Default Method
After completing the system installation steps (Section 2),
preparing the sample, and inserting the column, you are ready
to perform a run with the default method. The Torrent’s
default methods are factory-set with run parameters typically
used by chemists. Default methods are optimized for RediSep
Rf columns. The default settings will:
• collect all fluid in the fraction collector rack
• differentiate between UV (254 nm) vs. non-UV absorbing
fluid and place the fluids into separate tubes
• cut UV absorbing peaks based on slope detection or level
threshold.
To start the run from the PeakTrak Main window:
1. Enter a sample name. If you choose not to, PeakTrak will
enter the date and time as the sample name when you start
the run.
2. Review the PeakTrak Main window settings. If you want to
change any settings, refer to the Editing a Method on-line
help topic.
3. Click the Play button.
4. Select the sample Loading Type from the list. This Loading
Type should support the sample preparation you chose in
Section 3.2. Possible sample loading types are:
• Solid (Pause) – Select this option if you intend place
the sample into a solid sample load cartridge, but
have not yet prepared the sample. When you click the
run button, the system will perform a column
equilibration and then wait while you prepare the
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