CombiFlash Torrent® User Manual - Isco

Section 3 Operation
3.3 Loading a RediSep Rf Column
To load a RediSep Rf column:
WARNING
The CombiFlash Torrent has redundant safety devices to limit
pressure to less than 100 psi (689 kPa). RediSep columns
smaller than 100 g are CE certified using standard IEC61010-1
for use on the CombiFlash Torrent. RediSep columns larger
than 100 g meet Pressure Vessel Directive 97/23/EC. Teledyne
Isco strongly recommends against the use of columns rated
less than 100 psi (689 kPa).
Note
For best results, always use RediSep Rf columns. The system will
not automatically detect other columns, including non-Rf RediSep
columns. The system also limits the maximum operating pressure
to 50 psi when the column is not detected.
1. Select a RediSep Rf column. The on-line help includes a
column selection guide that can assist you in selecting a
stationary phase media and column size.
CAUTION
Pinch point. Your fingers or hands can be seriously
injured if you place them between the moving parts of
the Solid Load Cartridge Cap Mount or the Upper
Column Mount. Use care when installing solid load
cartridges or columns.
2. Raise the upper column mount until it is held by the latch.
3. Insert the column onto the lower column mount.
4. Firmly grasp the upper column mount, raise it, and then
push the latch to the left to release it.
5. Slowly lower the column mount and align it with the
column inlet. The spring-loaded mount will secure the
column in place.
6. Rotate the column 1 4 turn to seal the fittings.
3-9