CombiFlash Torrent® User Manual - Isco
CombiFlash Torrent® User Manual - Isco
CombiFlash Torrent® User Manual - Isco
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Section 3 Operation<br />
3.3 Loading a RediSep Rf Column<br />
To load a RediSep Rf column:<br />
WARNING<br />
The <strong>CombiFlash</strong> Torrent has redundant safety devices to limit<br />
pressure to less than 100 psi (689 kPa). RediSep columns<br />
smaller than 100 g are CE certified using standard IEC61010-1<br />
for use on the <strong>CombiFlash</strong> Torrent. RediSep columns larger<br />
than 100 g meet Pressure Vessel Directive 97/23/EC. Teledyne<br />
<strong>Isco</strong> strongly recommends against the use of columns rated<br />
less than 100 psi (689 kPa).<br />
Note<br />
For best results, always use RediSep Rf columns. The system will<br />
not automatically detect other columns, including non-Rf RediSep<br />
columns. The system also limits the maximum operating pressure<br />
to 50 psi when the column is not detected.<br />
1. Select a RediSep Rf column. The on-line help includes a<br />
column selection guide that can assist you in selecting a<br />
stationary phase media and column size.<br />
CAUTION<br />
Pinch point. Your fingers or hands can be seriously<br />
injured if you place them between the moving parts of<br />
the Solid Load Cartridge Cap Mount or the Upper<br />
Column Mount. Use care when installing solid load<br />
cartridges or columns.<br />
2. Raise the upper column mount until it is held by the latch.<br />
3. Insert the column onto the lower column mount.<br />
4. Firmly grasp the upper column mount, raise it, and then<br />
push the latch to the left to release it.<br />
5. Slowly lower the column mount and align it with the<br />
column inlet. The spring-loaded mount will secure the<br />
column in place.<br />
6. Rotate the column 1 4 turn to seal the fittings.<br />
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