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CombiFlash Torrent® User Manual - Isco

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Section 3 Operation<br />

1<br />

2<br />

Figure 3-3 Press the lever and extend the plunger<br />

3. Slide the plunger into the cartridge until it reaches the top<br />

frit (Figure 3-4).<br />

4. Press the lever and push the cartridge into the cap. Align<br />

the cartridge so that it fits fully into the recess in the cap<br />

(Figure 3-5).<br />

5. Load the solid sample cartridge with cap on the sample<br />

injection port.<br />

CAUTION<br />

Pinch point. Your fingers or hands can be seriously<br />

injured if you place them between the moving parts of<br />

the Solid Load Cartridge Cap Mount or the Upper<br />

Column Mount. Use care when installing solid load<br />

cartridges or columns.<br />

3.2.3 Preloading on Column<br />

You can load the reaction mixture or sample directly onto the<br />

column, sometimes called “direct” or “dry” loading.<br />

Note<br />

If using this method, use care to ensure that a column equilibration<br />

does not wash away the sample. Bypass the column equilibration<br />

at run time by selecting “Preload on column” as the sample<br />

Loading Type. Refer to Section 3.4 for more details.<br />

3-7

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