CombiFlash Torrent® User Manual - Isco
CombiFlash Torrent® User Manual - Isco
CombiFlash Torrent® User Manual - Isco
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<strong>CombiFlash</strong> Torrent® <strong>User</strong> <strong>Manual</strong><br />
c. Agitate the solvent for a moment to allow the sample to<br />
adsorb to the silica.<br />
d. Remove the solvent with a suitable method, such as<br />
rotary evaporation. Alternatively, you can dry the<br />
prepared cartridge after step 5 by drawing the solvent<br />
out using a vacuum or using the Torrent’s air purge.<br />
3. Load the media and sample mixture into the cartridge. Tap<br />
the cartridge on the benchtop to settle the mixture.<br />
4. Place a frit on the top of the cartridge. Force the frit down<br />
against the mixture using the plastic plunger.<br />
5. Wipe away any residual powder inside the neck and top<br />
rim of the cartridge.<br />
Note<br />
You can remove solvent from a prepared cartridge by attaching the<br />
cartridge cap and manually purging (TOOLS>MANUAL CONTROL)<br />
the cartridge with air.<br />
Note<br />
Empty solid sample cartridges also allow a variety of adsorbents,<br />
such as diatomaceous earth, boiling chips, cotton balls, or paper<br />
wipes. Even with alternative adsorbents, always use top and<br />
bottom frits to prevent fluid path problems.<br />
After you have prepared the pre-filled or empty cartridge,<br />
place the solid sample cartridge on the system:<br />
1. Attach the desired adjustable cartridge cap:<br />
• 60-5237-048, fits 12 and 20 gram solid load<br />
cartridges.<br />
• 60-5237-044, fits 32 and 65 gram solid load<br />
cartridges.<br />
• 60-5247-008, fits 125 and 260 gram solid load<br />
cartridges.<br />
• 60-5247-009, fits 750 gram solid load cartridges.<br />
2. Press the lever on the side of the cap and fully extend the<br />
plunger (Figure 3-3).<br />
3-6