CombiFlash TorrentÂ® User Manual - Isco

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CombiFlash Torrent® User Manual The Direct Injection Adapter is recommended when using the Liquid Load Pump to load samples directly on the column, or when there is a need to bypass the automated injection valve during sample injections. 3.1.4 Liquid Injection Adapter The Liquid Injection Adapter (60-5244-059) should be used when performing liquid sample injections with a syringe. The valve prevents the liquid from be forced back out of the injection port. Refer to section 3.4.1 for more information. 3.1.5 Prime Tube Assembly The prime tube assembly (209-0165-76), also called a column bypass tube, can be used in place of a column when purging/priming the system and when performing maintenance. 3.1.6 Prime Pump Plug, 1/8-inch Use this plug (209-0165-21) to cap the B Solvent’s Prime port when this port is not in use. 3.1.7 Stylus The stylus with adhesive-backed mounting plate may be attached near the LCD display for precise control of the touch screen. 3.1.8 Test Mix B Vials and 5 mL Syringe Test Mix B can be used to determine if the system is operating correctly. Refer to SYSTEM VERIFICATION WITH TEST MIX B in section 2.15 for instructions. 3.1.9 Cable Clips and Ties Use the clips and ties to secure tubing or wiring after installation. 3.2 Sample Preparation Before starting a run, consider how the sample will be introduced to the column media. This section discusses three methods: liquid injection, solid sample cartridges, and preloading the sample on the column. 3-4
Section 3 Operation 3.2.1 Liquid Sample Injection If the sample is soluble in the starting mobile phase, it can be prepared as a solution and injected onto the column when prompted during the purification run. 3.2.2 Solid Samples Some compounds are not soluble in solvents that are compatible with the chromatography. In addition, the compounds may have very limited solubility in any solvent, resulting in sample volumes that are impractical for good chromatography. The answer in this case is solid sample introduction. Here the reaction mixture or dissolved sample is mixed with the same media in the chromatography column. Some samples of this type may be simply dissolved and placed into a prefilled cartridge. You can then use the cartridge immediately or dry it before placing it on the system. Other samples may need more care. Typically you would create a mixture of 20% sample load to media (w/w). Then dry the mixture under conditions that will drive off the solvent without affecting the compounds of interest. These compounds remain bound to the media. Once dried, pour the media/sample mixture into an empty cartridge. To prepare an empty solid sample cartridge: 1. Ensure the empty cartridge has a bottom frit. CAUTION Missing frits may cause equipment damage, UV detection problems, or increased maintenance. Frits prevent solids from entering the fluid path. 2. Prepare the media and sample: a. Dissolve your sample in a minimal amount of a suitable, volatile solvent. b. Place the media into the solvent. If using silica gel, a particle size of 40–60 µm (240–400 mesh) is recommended. The amount of silica required is about four to five times the mass of your sample. 3-5
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For indoor use only CombiFlash Torr
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CombiFlash Torrent ® User Manual T
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Table of Contents 4.2 PeakTrak Menu
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CombiFlash Torrent ® User Manual S
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Section 1 Introduction 1.3 Operatin
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Section 1 Introduction Table 1-1 Co
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Section 1 Introduction media of cho
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Section 4 User Help Reference 4.4.7
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Section 6 Maintenance 6.3 Cleaning
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Section 6 Maintenance 6.6 Electrica
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DECLARATION OF CONFORMITY Applicati
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