07.03.2014 Views

CombiFlash Torrent® User Manual - Isco

CombiFlash Torrent® User Manual - Isco

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Section 2 Preparation<br />

Foxy 3 Delay Volume – 60 mL<br />

Foxy 4 Delay Volume – 80 mL<br />

Fractionation Valve Delay Volume – 10 mL<br />

If using custom-length tubing, calculate the delay volume of<br />

all tubing between the Torrent’s TO COLLECTOR port and<br />

the diverter valve inlet using the formula:<br />

V = / 4 D 2 L<br />

(otherwise expressed as: V= 0.7854 D 2 L),<br />

where L is the tubing length (cm) and D is the inside diameter<br />

(cm). The resulting volume is in milliliters.<br />

Fractionation Valve Configuration – If using the<br />

Fractionation Valve, enable the Fractionation Valve option<br />

and enter a delay volume. The delay volume entries ensure<br />

that the tube changes occur when the peak fluids are present<br />

at the fraction collector diverter valve. You can calculate the<br />

delay volume using the formula above.<br />

2.13.4 Restart the system<br />

Some configuration settings do not take effect until the system<br />

is restarted. After completing sections 2.13.1 through 2.13.3<br />

restart the system:<br />

1. If the Configuration window is not closed already, click the<br />

OK button to close it.<br />

2. Place the Power switch below the LCD display in the Off<br />

position and wait one minute or more.<br />

CAUTION<br />

Always wait at least one minute after placing the Power switch in<br />

the Off position. Moving the switch to the Off position begins a<br />

power down sequence that takes up to one minute to complete. If<br />

you move the switch to the ON position before the sequence is<br />

complete, the system may not start up correctly.<br />

2-35

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