CombiFlash Torrent® User Manual - Isco

CombiFlash Torrent® User Manual
Automatically Print Report at End of Run – if this option
is enabled, the system will print a report at the end of each
run.
Note
This option requires the system to be configured for network
operation and a connection to a printer on the network. Network
settings are discussed in Section 5, REMOTE INTERFACES. Do not
select this option for these initial installation steps.
Beeper – If enabled, the Torrent will sound an alert whenever
user intervention is required during a run.
Default Run Units – Run units are displayed along the
X-axis of the chromatogram. Select Time (in minutes) or
Column Volumes. A column volume unit is the time it takes to
pump enough solvent to exchange the volume held by the
column. The duration in minutes will vary according to the
column media and size, and the flow rate.
Gradient Method – Select the standard gradient type
(Figure 2-20).
Linear
Figure 2-20 Gradient types
Step
Isocratic
• Linear – The system gradually mixes solvents A and B
from the last programmed %B until it reaches the next
programmed %B. This method connects the inflection
points that define the gradient with a straight line.
• Step – The system holds each %B value until it reaches
the next programmed %B. Because the system holds the
%B values, the changes at each new value are nearly
instantaneous, producing a stepped appearance on the
plot area.
• Isocratic – The system holds the initial solvent mix for
the entire run.
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