CombiFlash Torrent® User Manual - Isco

CombiFlash Torrent® User Manual
6.5.3 Monthly Flow Cell Cleaning
Perform this procedure as part of your scheduled preventive
maintenance, or when Quick Cleaning As Required (section
6.5.2) does not improve the lamp energy.
1. Remove the column and insert a bypass tube between the
upper and lower column mounts. A bypass tube
(209-0165-46) can be found in the accessory kit
(60-5239-006).
2. From the menu, select Tools>Manual Control. This opens
the Manual Control window.
3. Set the Flow Rate to 200 mL/min (Figure 6-4).
4. Select Through column for the Valve Position option.
5. Select Next for the Pump into Tube # option.
6. Place the B1 Solvent inlet line into a reservoir of methanol,
acetone, or a strong solvent that readily dissolves residual
sample material.
7. Click the Prime B button to pump 100% Solvent B through
the bypass tube and into the collection tubes.
8. After three minutes, click the Stop button. Allow the
system to stand for at least six hours. Overnight is
recommended.
9. Return the B1 solvent line to the original solvent container.
10.Perform the Quick Cleaning as Required (section 6.5.2) and
monitor the Raw Lamp Energy.
If the lamp energy is in the green or yellow range, return
the system to operation. If the lamp energy is red, contact
Teledyne Isco’s Technical Service department for assistance.
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