CombiFlash TorrentÂ® User Manual - Isco

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CombiFlash Torrent® User Manual Note Pumping solvent B at a moderate flow rate (150 to 200 mL/min) over time will usually solubilize obstructions. Generally, the recommended solvent is the highest polarity solvent you have recently used (solvent B). 8. Monitor the Raw Lamp Energy gauge. As the system pumps solvent, the raw lamp energy should gradually improve. Pump solvent for two to five minutes or until the indicator reaches the far-right of the green range. When the indicator is in the yellow, the lamp energy and flow cell are ready for the next run. However, continuing the cleaning for greater raw lamp energy should reduce the frequency of flow cell cleaning messages (Figure 6-2). If after five minutes the indicator is still in the red range, repeat the cleaning steps using an alternative solvent (Table 6-1). Or, complete the steps in the Monthly Flow Cell Cleaning procedure (section 6.5.3). Note If the numerical values of the Raw Lamp Energy (Figure 6-3) do not change, or if the first number remains at zero, contact Teledyne Isco’s Technical Service department. 6-8
Section 6 Maintenance Solvent Methanol Acetone Dichloromethane (DCM) Table 6-1 Suggested Flow Cell Cleaning Solvents a Dimethylsulfoxide (DMSO) Dimethylformamide (DMF) Tetrahydrofuran (THF) Water/DMF/ methanol Water/DMF/ Acetic Acid Mildly acidic aqueous solution Mildly basic aqueous solution Description Miscible with most LC solvents. Methanol will force other solvents through the system so they will not interfere with the next sample. It breaks down the tertiary structure of silica and allows it to flush through. Common organic solvent with high dissolving properties. Acetone absorbs UV light at 254 nm. Therefore, the Raw Lamp Energy gauge will always read red when acetone is in the flow cell. After pumping acetone, pump a different solvent through the system to remove the acetone and read the Raw Lamp Energy gauge. Effective at solubilizing organic molecules. Strong solvent that will clear organic sample. Strong solvent if other solvents do not produce the desired results. Strong solvent. A 50/25/25 solvent mixture effective in clearing some obstructions. 30/30/30ml solvent mixture effective in clearing some obstructions. 0.5M acetic acid in distilled water can be used to clear samples that are not removed by organic solvents. A dish-type detergent solution can wash through particulates not picked up by the acidic solution. The emulsification effect of detergent is an effective tool as well to help wash through oily samples. Follow the detergent with several minutes of clean, distilled water. a. Recent synthesis solvents should also be considered. 6-9
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For indoor use only CombiFlash Torr
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CombiFlash Torrent ® User Manual T
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Table of Contents 4.2 PeakTrak Menu
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CombiFlash Torrent ® User Manual S
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Section 1 Introduction 1.3 Operatin
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Section 1 Introduction Table 1-1 Co
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Section 1 Introduction media of cho
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Section 1 Introduction 1.6.1 Hazard
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Section 1 Introduction Table 1-2 Ha
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Section 2 Preparation quickly be re
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Section 2 Preparation CAUTION Ensur
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Section 2 Preparation a. Locate a n
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Section 2 Preparation CAUTION Eleva
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Section 2 Preparation If an optiona
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Section 2 Preparation 2.10.2 Option
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Section 2 Preparation TCP/IP Settin
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Section 2 Preparation Torrent/Foxy
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Section 2 Preparation In Example 3,
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Section 2 Preparation 2. Connect th
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Section 2 Preparation 2.10.4 Model
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Section 2 Preparation 2. Using the
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Section 2 Preparation Union Figure
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Section 2 Preparation (sections 2.1
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Section 2 Preparation Foxy 3 Delay
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Section 2 Preparation Step Table 2-
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Section 3 Operation Knurled Cap Car
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Section 3 Operation 3.2.1 Liquid Sa
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Section 3 Operation 3.5 During the
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Section 4 User Help Reference 4.1.2
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