CombiFlash Torrent® User Manual - Isco

CombiFlash Torrent® User Manual
Note
Pumping solvent B at a moderate flow rate (150 to 200 mL/min)
over time will usually solubilize obstructions. Generally, the
recommended solvent is the highest polarity solvent you have
recently used (solvent B).
8. Monitor the Raw Lamp Energy gauge. As the system
pumps solvent, the raw lamp energy should gradually
improve. Pump solvent for two to five minutes or until the
indicator reaches the far-right of the green range.
When the indicator is in the yellow, the lamp energy and flow
cell are ready for the next run. However, continuing the
cleaning for greater raw lamp energy should reduce the
frequency of flow cell cleaning messages (Figure 6-2).
If after five minutes the indicator is still in the red range,
repeat the cleaning steps using an alternative solvent
(Table 6-1). Or, complete the steps in the Monthly Flow Cell
Cleaning procedure (section 6.5.3).
Note
If the numerical values of the Raw Lamp Energy (Figure 6-3) do
not change, or if the first number remains at zero, contact
Teledyne Isco’s Technical Service department.
6-8