CombiFlash Torrent® User Manual - Isco
CombiFlash Torrent® User Manual - Isco
CombiFlash Torrent® User Manual - Isco
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<strong>CombiFlash</strong> Torrent® <strong>User</strong> <strong>Manual</strong><br />
CAUTION<br />
When using methanol as solvent B with silica column media, do<br />
not pump more than 50% solvent B. Higher percentages of<br />
methanol might break down the silica structure, possibly causing<br />
obstructions in the flow path.<br />
6.5.2 Quick Cleaning when Recommended<br />
When the lamp energy is lower than normal, the system will<br />
recommend flow cell cleaning (Figure 6-2) before starting a<br />
separation run.<br />
When the system displays this message you can:<br />
• Cancel Run (recommended) – Click the Cancel Run<br />
button so you can perform a quick cleaning described in<br />
the following steps.<br />
• Continue Collect All – Click this button to ignore the<br />
message. Because the peak detection operation might be<br />
impaired, the system automatically collects all fluids to<br />
avoid diverting compounds of interest to waste.<br />
• Help – Click this button to display the flow cell cleaning<br />
on-line help topic.<br />
To perform a quick cleaning:<br />
1. After clicking the Cancel Run button, select the<br />
Tools><strong>Manual</strong> Control menu option.<br />
2. From the <strong>Manual</strong> Control window (Figure 6-3), note the<br />
Raw Lamp Energy level at 254 nm. The Raw Lamp Energy<br />
gauge has three ranges: red, yellow, and green.<br />
Figure 6-3 Raw Lamp Energy Gauge<br />
6-6