CombiFlash Torrent® User Manual - Isco

Section 6 Maintenance
End of
Separation
Flush
100
Separation
Gradient
Initial %B Next Run Initial %B
End of
Run
75
50
25
0
Gradient % Solvent B
Time or Column Volumes
Figure 6-1 Default post-separation column and flow cell flush
Figure 6-2 Flow cell cleaning message
Typically, chemists Stop and then Fast Forward the run after
the last compound elutes. This action skips the
post-separation flush. If any of the above conditions appear,
consider allowing some of the runs to continue through the
flush, or run a high percentage of %B solvent through the
system for a few minutes at the end of each day.
If the separation runs always continue through the flush and
the conditions still occur frequently, edit the default column
methods to extend the flush duration.
6-5