CombiFlash Torrent® User Manual - Isco

CombiFlash Torrent® User Manual
6.4 Tubing Inspection
WARNING
Risk of fire or equipment damage. Faulty tubing, fittings, and
drains may allow organic solvents to pool in unsafe areas,
creating a potential for dangerous levels of flammable vapors.
Improper draining may damage the instrument’s internal
components.
Perform a tubing inspection monthly:
1. Visually inspect the solvent, waste, and drain tubing. The
tubing must be free of any damage, kinks, or deterioration.
Fittings should show no signs of leaks.
2. Test the drip tray drain by connecting a vacuum or air
supply source to the outlet end of the drain tube. Then,
verify the presence of such vacuum or air supply source on
the drain hole on the drip tray.
Correct any deficiencies before returning the instrument to
operation.
6.5 Flow Cell Cleaning
6.5.1 Post Separation
As a preventive measure, all default column methods finish
the separation run with a high percentage of solvent B
(Figure 6-1). This brief time (two to three column volumes) of
strong solvent flushes residual compounds from the column,
flow cell, and internal tubing.
Skipping the post-separation flush may cause residual
compounds to build up and crystallize, which might result in:
• cross contaminating later separation runs
• higher operating pressures
• reduced flow cell lamp energy
• a noisy baseline on the absorbance trace
• frequent messages recommending flow cell cleaning
(Figure 6-2).
6-4