CombiFlash Torrent® User Manual - Isco

Section 4 User Help Reference
for modules with flow cell windows stained by
compounds.
• Red – lamp energy is obstructed to a degree that the
module might not reliably detect peaks. If you attempt to
operate the module, peak collection will be forced to
collect all. This prevents diverting desired compounds to
waste.
Depending on what you are doing with the Manual Control
functions, low lamp energy could be a normal indication. For
example, a UV-absorbing compound could be present in the
flow cell as you are pumping solvent. Or, the selected solvent
absorbs UV light at 254 nm. Abnormal indications could be a
flow cell blocked by a compound that has precipitated or there
is a film built up on the flow cell. If you suspect the low lamp
energy is an abnormal indication, perform the steps in the
QUICK CLEANING WHEN RECOMMENDED [ON-LINE] topic.
4.4.11 Setting the Vapor Limit
The module has an internal vapor sensor that detects vapors
present in the module. When the limit is exceeded, the
CombiFlash Torrent module will shut down to avoid a
hazardous condition.
Teledyne Isco recommends using the default setting of 12.
This value represents an organic vapor level slightly above the
ambient vapor level of a well-ventilated laboratory.
A setting below 12 could cause random alarms without
significant vapors present. If PeakTrak displays a Vapor Limit
alarm while the Vapor Limit is set at 12, perform the following
checks on your laboratory and the instrument:
• Ensure that no open containers or spills of organic
solvent are in close proximity to the module.
•Ensure that the module is located in a well-ventilated
area.
• Ensure that there is no visible solvent leakage from the
module.
If PeakTrak continues to display the Vapor Limit alarm after
you have made these checks and corrected any problems
found, it is likely that organic vapors are present in the
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