CombiFlash Torrent® User Manual - Isco

CombiFlash Torrent® User Manual
module, first open the MANUAL CONTROL [4.3.9] window by
selecting the Tools>Manual Control menu command.
4.4.10.1 Pumping Solvents
To pump either solvent, select the solvent from the Solvent A
or Solvent B lists. Then, click the Prime A or Prime B buttons
to pump the selected solvent. To pump a mixture or Solvent A
and B, adjust the Percentage Solvent B setting and then click
the Prime __% B button. When finished, click the Stop button.
You can control the flow rate by adjusting the Flow Rate
mL/min setting.
By default, the module pumps the solvent directly to the waste
port. You can change the fluid path by selecting a different
Valve Position option (through column or through cartridge
and column). For valve positions other than Direct To Waste,
you can pump the solvent into a collection tube. To do so, select
the Pump Into Tube # option and then select which tube
(“next” or specific tube number).
4.4.10.2 Air Purging
You can purge the solid sample cartridge and column path
with air. To air purge a path, click the Air Purge Solid Sample
or Air Purge Column buttons. Click the Stop button to halt the
air purge.
4.4.10.3 Raw Lamp Energy
The right side of the Manual Control window shows a Raw
Lamp Energy gauge. This provides an indication of the UV
light measured by the optical detector at 254 nm. High lamp
energy (green) means that the flow cell easily passes through
a sufficient UV light source. Lower lamp energy (yellow or red)
means that either the light source is weak or that the flow cell
is obstructed.
• Green – lamp energy is sufficient to detect peaks up to
3.0 Absorbance Units.
• Yellow – lamp energy is partially obstructed and the
peak detector performance might be impaired. The
module can be operated although the absorbance trace
might appear noisy. Yellow might be a normal indication
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