CombiFlash Torrent® User Manual - Isco
CombiFlash Torrent® User Manual - Isco
CombiFlash Torrent® User Manual - Isco
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<strong>CombiFlash</strong> Torrent® <strong>User</strong> <strong>Manual</strong><br />
module, first open the MANUAL CONTROL [4.3.9] window by<br />
selecting the Tools><strong>Manual</strong> Control menu command.<br />
4.4.10.1 Pumping Solvents<br />
To pump either solvent, select the solvent from the Solvent A<br />
or Solvent B lists. Then, click the Prime A or Prime B buttons<br />
to pump the selected solvent. To pump a mixture or Solvent A<br />
and B, adjust the Percentage Solvent B setting and then click<br />
the Prime __% B button. When finished, click the Stop button.<br />
You can control the flow rate by adjusting the Flow Rate<br />
mL/min setting.<br />
By default, the module pumps the solvent directly to the waste<br />
port. You can change the fluid path by selecting a different<br />
Valve Position option (through column or through cartridge<br />
and column). For valve positions other than Direct To Waste,<br />
you can pump the solvent into a collection tube. To do so, select<br />
the Pump Into Tube # option and then select which tube<br />
(“next” or specific tube number).<br />
4.4.10.2 Air Purging<br />
You can purge the solid sample cartridge and column path<br />
with air. To air purge a path, click the Air Purge Solid Sample<br />
or Air Purge Column buttons. Click the Stop button to halt the<br />
air purge.<br />
4.4.10.3 Raw Lamp Energy<br />
The right side of the <strong>Manual</strong> Control window shows a Raw<br />
Lamp Energy gauge. This provides an indication of the UV<br />
light measured by the optical detector at 254 nm. High lamp<br />
energy (green) means that the flow cell easily passes through<br />
a sufficient UV light source. Lower lamp energy (yellow or red)<br />
means that either the light source is weak or that the flow cell<br />
is obstructed.<br />
• Green – lamp energy is sufficient to detect peaks up to<br />
3.0 Absorbance Units.<br />
• Yellow – lamp energy is partially obstructed and the<br />
peak detector performance might be impaired. The<br />
module can be operated although the absorbance trace<br />
might appear noisy. Yellow might be a normal indication<br />
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