CombiFlash Torrent® User Manual - Isco

Section 4 User Help Reference
• Close button — Click the Close button when you are
done viewing the run.
4.4.9.1 General instructions for reading the data
Most elements on the window are color-keyed to help you
locate the tubes containing the peaks of interest. Below each
collected peak there is a color bar that matches a tube in the
map on the left. The tube map provides a visual
representation of the tubes that contain the peaks of interest.
If you wish to identify the tube by number, refer to the table
below the rack diagram.
If the run used multiple Foxy fraction collector racks, or
multiple Fractionation Valve bottle sets (also considered
racks), the Previous and Next Rack buttons are active. Click
these buttons to scroll through the available racks. The
number of the currently displayed rack is shown at the top left
of the window.
4.4.9.2 Reading the Chromatogram
The plot area displays the following:
• The red absorbance trace produced by the module’s peak
detector. Absorbance units (also shown in red) that
correspond to this trace are shown on the left Y-axis.
• Purple absorbance and green ratio traces may be visible
if you are monitoring a second wavelength. See the
MONITORING THE WAVELENGTH RATIO [4.4.7] help topic.
• The gradient curve that was used during the run. The %
Solvent B scale is shown in blue on the right Y-axis.
• The X-axis depicts the run time, shown as minutes or
COLUMN VOLUMES [ON-LINE].
• Vertical lines may appear at intervals along the X-axis.
These lines indicate collection tube changes. To prevent
the plot area from being obscured by tube change marks,
PeakTrak may limit the number of marks.
4.4.10 Manual Control of the CombiFlash Torrent Module
Manual Control can assist with method development,
maintenance of the module plumbing, and system
troubleshooting. To manually control the CombiFlash Torrent
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