CombiFlash TorrentÂ® User Manual - Isco

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CombiFlash Torrent® User Manual • Chromatogram — The right side of the window displays the chromatogram, identified by the Sample Name in the title bar of the window. • Option buttons — The option buttons give you quick access to frequently used commands while viewing a run. The buttons are: • Print — Click this button to print a run summary. When viewing the run from a remote personal computer, the summary can be printed on any installed printer. If you are attempting to print from the module’s touch panel display, you must first set up NETWORK PRINTING in the NETWORK CONFIGURATION [4.3.10.2] settings. • Save As PDF — Click this button to save the run summary in PDF file format. When viewing the run from a remote personal computer, the summary can be saved to any connected storage device. If you are attempting to save a PDF file from the touch panel display, insert a USB Flash drive in the USB port below the display panel. • Save As TXT — Click this button to save the run summary in an ASCII text file format. When viewing the run from a remote personal computer, the summary can be saved to any connected storage device. If you are attempting to save a TXT file from the touch panel display, insert a USB Flash drive in the USB port below the display panel. • Zoom In — Click this button to enable the zoom feature. Then, click and drag across the region to be magnified. • Zoom Out — Click this button to restore the chromatogram to the normal view. • Format Graph — Click this button to open a window from which you can set the X and Y-axis scales. • Extract Method button — Click the Extract button to load a new method file based on the parameters for the run you are viewing. 4-48
Section 4 User Help Reference • Close button — Click the Close button when you are done viewing the run. 4.4.9.1 General instructions for reading the data Most elements on the window are color-keyed to help you locate the tubes containing the peaks of interest. Below each collected peak there is a color bar that matches a tube in the map on the left. The tube map provides a visual representation of the tubes that contain the peaks of interest. If you wish to identify the tube by number, refer to the table below the rack diagram. If the run used multiple Foxy fraction collector racks, or multiple Fractionation Valve bottle sets (also considered racks), the Previous and Next Rack buttons are active. Click these buttons to scroll through the available racks. The number of the currently displayed rack is shown at the top left of the window. 4.4.9.2 Reading the Chromatogram The plot area displays the following: • The red absorbance trace produced by the module’s peak detector. Absorbance units (also shown in red) that correspond to this trace are shown on the left Y-axis. • Purple absorbance and green ratio traces may be visible if you are monitoring a second wavelength. See the MONITORING THE WAVELENGTH RATIO [4.4.7] help topic. • The gradient curve that was used during the run. The % Solvent B scale is shown in blue on the right Y-axis. • The X-axis depicts the run time, shown as minutes or COLUMN VOLUMES [ON-LINE]. • Vertical lines may appear at intervals along the X-axis. These lines indicate collection tube changes. To prevent the plot area from being obscured by tube change marks, PeakTrak may limit the number of marks. 4.4.10 Manual Control of the CombiFlash Torrent Module Manual Control can assist with method development, maintenance of the module plumbing, and system troubleshooting. To manually control the CombiFlash Torrent 4-49
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For indoor use only CombiFlash Torr
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CombiFlash Torrent ® User Manual T
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Table of Contents 4.2 PeakTrak Menu
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