CombiFlash Torrent® User Manual - Isco

Section 4 User Help Reference
TLC, wait at least the time established as T. After this time
has elapsed it is likely that the desired compound has come
off the cartridge and you can safely click the Cartridge
Bypass button.
For example, a TLC plate run at 30%B revealed that the last
desired compound had an Rf factor of 0.35. An empty 25-gram
size solid load cartridge was filled with sample adsorbed onto
10 grams of silica, and then placed on the system. The
programmed flow rate was 60 mL/min. During the run, the
flow rate dropped to 30 mL/min. The equation is then:
T = 45 mL / (0.35 * 30 mL/min) = 4.3 minutes
In this example, the Cartridge Bypass feature was used 4.3
minutes after the solvent gradient reached 30% B.
The calculation is conservative, but allows you to minimize the
time needed to keep the solid load cartridge in the fluid path.
4.4.9 Viewing runs
After completing a run, the PeakTrak Main window is used to
display all collected run data. You can also open previous runs
to view the chromatogram and the peak/tube locations. To
open a previous run:
1. From the main window, select File>Open, or click the Open
button. The FILES [4.3.5] window is displayed.
2.Choose a Run file and click the Open button.
3.PeakTrak displays the run in the MAIN WINDOW [4.3.1].
The PeakTrak Main window will include:
• Rack and tube information — The left pane of the
window lists the current rack, a map for that rack, and a
table that lists the peaks and their corresponding tube
numbers. If the window is currently displaying the
collection parameters, click the Rack View button to
view this information.
• Method parameters — Click the Display Method
button to view a summary of the peak detection and
collection settings for the run.
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