CombiFlash Torrent® User Manual - Isco

CombiFlash Torrent® User Manual
button. The settings will revert to the original factory settings.
Click the Save button to save the settings for future use.
4.4.5 Defining a Gradient
The simplest way to change the gradient is to click and drag
the inflection points that define the shape of the gradient. Add
a point by clicking the Insert button, then click the gradient
curve to add a point. You can then drag the new point to the
desired location. Delete a point by clicking the Delete button
and then clicking the undesired inflection point.
The above methods work in the MAIN WINDOW [4.3.1] and
METHOD EDITOR [4.3.2] window. Alternatively, you can modify,
add, insert, and delete points using a tabular view on the
Method Editor window. Click the Edit Gradient button to open
this view, then use the controls to modify the settings. Click
the Edit Gradient button again to close the table view.
PeakTrak can also optimize a gradient from your TLC results.
To use this feature, select a single solvent system and run two
TLC plates, each with a different concentration of solvent. For
example, run one plate at 1:1 hexane:ethyl acetate, the other
at 1:3 hexane:ethyl acetate. If the resulting Rf values are
between 0.2 and 0.8, you can enter the TLC data in the
GRADIENT OPTIMIZER [4.3.3] window. When you accept the data,
PeakTrak will generate a gradient curve to separate the target
compound from the nearest impurity. When using optimized
gradients, PeakTrak may automatically insert an isocratic
hold to further increase the resolution.
4.4.6 Real-time Gradient Editing
The gradient shape can be changed during the run. Any time
after the fraction collector has positioned the drop former over
the first tube, click and drag the points on the gradient plot
area of the Main window. You can also add or remove points by
clicking the Insert or Delete point buttons. The Insert button
allows you to add a single point when you click on the gradient
profile. Repeat this action to add more points. The Delete
button allows you to remove a single point when you click on
it.
Note that only the portion of the gradient that has not yet
occurred during the run can be modified.
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