CombiFlash Torrent® User Manual - Isco

Section 4 User Help Reference
save the method using the FILE>Save or Save As menu
commands.
4.4.3.3 Scaling Method Files to Different Column Sizes
If you want to scale a method file to a different column size,
use the FILE>Save As menu command. From the FILES [4.3.5]
window, select the Scale option, then choose the new column
size and click the Save button. The module will create a new
method file with the gradient and run parameters scaled to
the new column size.
This feature is useful when developing methods using small
sample volumes. After you optimize the method to successfully
extract the compounds of interest, you can then scale up the
method to load and extract larger volumes using larger
RediSep column sizes.
4.4.4 Editing a Default Method
Default Methods allow you to quickly recall standard method
settings. There are standard methods for each size and type of
RediSep column. When shipped from the factory, the Default
Methods were set to the settings recommended by Teledyne
Isco.
The Default Method settings can be tailored to your
application. To modify the Default Methods:
1. Select the TOOLS>EDIT DEFAULTS [4.2.3.5] menu command.
2.If prompted for a password, enter the module password.
(The SET PASSWORD [4.3.11] window controls the password
protection feature.)
3.Select the column size and media from the RediSep Column
list.
4.Change the settings to meet your specific application
requirements. Refer to the EDITING A METHOD [4.4.2]
instructions.
5.Click the Save button (or File>Save) to replace the default
method settings.
You can reset the method to Teledyne Isco-recommended
defaults. While viewing the default method file for a selected
column size and type, click the “Reset file to Isco defaults”
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