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CombiFlash Torrent® User Manual - Isco

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Section 4 <strong>User</strong> Help Reference<br />

• Peak Width — Select the average peak width<br />

setting. Peak widths are measured at the baseline.<br />

The slope detector will detect peak widths ranging<br />

from about 0.2 to 2 times the peak width setting. For<br />

example, if you entered a peak width of 1 minute, the<br />

range would be 12 seconds to 2 minutes. For best<br />

operation, the peak width should be set to just over<br />

the average peak width being separated. For<br />

instance, if the average peak width is 45 seconds, you<br />

should enter a peak width of 1 minute. For most flash<br />

chromatography, 1 minute is a good starting point for<br />

a peak width.<br />

• Threshold Detection — Select this option to enable<br />

Threshold peak detection. When enabled, peaks will<br />

be recorded if the Absorbance Units (AU) value is<br />

exceeded.<br />

• Threshold — Type or select the Absorbance Units<br />

value to be used for Threshold detection.<br />

Note: If both Slope and Threshold peak detection<br />

methods are checked, PeakTrak considers a peak to<br />

be present when any one condition is met. This logical<br />

OR operator means that PeakTrak will cut a peak<br />

when either the Slope condition is true, or when the<br />

Threshold condition is true.<br />

• All Wavelength Detection — Enable this option to<br />

detect peaks within a user-selected range of<br />

wavelengths. When enabled, click the Details button to<br />

configure additional settings. These settings include the<br />

slope-based and peak width options described in 1 and<br />

2 above. Additionally, you can type or select the<br />

minimum and maximum wavelengths limits in<br />

nanometers.<br />

• External Detector — Enable this option to use a 0 to 1<br />

volt analog input signal from an external detector. When<br />

enabled, click the Details button to configure additional<br />

settings. These settings include the slope-based and<br />

peak width options described in 1 and 2 above.<br />

PeakTrak will use its internal algorithms to cut detect<br />

and cut peaks based on the analog input signal. Refer to<br />

4-41

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