CombiFlash Torrent® User Manual - Isco
CombiFlash Torrent® User Manual - Isco
CombiFlash Torrent® User Manual - Isco
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Section 4 <strong>User</strong> Help Reference<br />
• Peak Width — Select the average peak width<br />
setting. Peak widths are measured at the baseline.<br />
The slope detector will detect peak widths ranging<br />
from about 0.2 to 2 times the peak width setting. For<br />
example, if you entered a peak width of 1 minute, the<br />
range would be 12 seconds to 2 minutes. For best<br />
operation, the peak width should be set to just over<br />
the average peak width being separated. For<br />
instance, if the average peak width is 45 seconds, you<br />
should enter a peak width of 1 minute. For most flash<br />
chromatography, 1 minute is a good starting point for<br />
a peak width.<br />
• Threshold Detection — Select this option to enable<br />
Threshold peak detection. When enabled, peaks will<br />
be recorded if the Absorbance Units (AU) value is<br />
exceeded.<br />
• Threshold — Type or select the Absorbance Units<br />
value to be used for Threshold detection.<br />
Note: If both Slope and Threshold peak detection<br />
methods are checked, PeakTrak considers a peak to<br />
be present when any one condition is met. This logical<br />
OR operator means that PeakTrak will cut a peak<br />
when either the Slope condition is true, or when the<br />
Threshold condition is true.<br />
• All Wavelength Detection — Enable this option to<br />
detect peaks within a user-selected range of<br />
wavelengths. When enabled, click the Details button to<br />
configure additional settings. These settings include the<br />
slope-based and peak width options described in 1 and<br />
2 above. Additionally, you can type or select the<br />
minimum and maximum wavelengths limits in<br />
nanometers.<br />
• External Detector — Enable this option to use a 0 to 1<br />
volt analog input signal from an external detector. When<br />
enabled, click the Details button to configure additional<br />
settings. These settings include the slope-based and<br />
peak width options described in 1 and 2 above.<br />
PeakTrak will use its internal algorithms to cut detect<br />
and cut peaks based on the analog input signal. Refer to<br />
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