CombiFlash TorrentÂ® User Manual - Isco

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CombiFlash Torrent® User Manual When using the All or Peaks options, you can specify the maximum Peak and Non-peak volumes to be collected in the tubes. Be sure to enter volumes less than the maximum tube capacity. Different peak and non-peak tube volumes can be used to conserve tubes when using the All peak collection mode by collecting large volumes of non-peak fluid while creating more, smaller fraction volumes of fluids of interest. The Foxy fraction collector or Fractionation Valve will advance to the next tube whenever a peak is detected. Also note that the fraction collector or valve will advance to the next tube if a new peak is detected before completing the last one (sometimes called a double advance). Keep in mind that the slope and threshold detection methods use algorithms optimize the peak detection. For example, peak detection includes a hysteresis to reduce the likelihood of multiple tube advances that may occur if there is noise at the beginning or tail end of a peak. These actions may be overridden if Time Windows are active. See the discussion on INITIAL WASTE & TIME WINDOWS. Peak Detection This section of the window contains option buttons to enable and disable various peak detection options. When an option is enabled, the window also allows you to modify the setting details for that peak detection option. All enabled options will be displayed on the chromatogram. Note: You can select up to two peak detection options (excluding the External option). If using two options, such as 1 with 2, PeakTrak considers a peak to be present when either option is true (a logical OR). • 1 and 2 (wavelength 1 and 2) — Enable these options to use and configure primary and secondary wavelength detection. When enabled, type or select the peak detection wavelength in nanometers, then click the Details button to configure additional settings described below. • Slope Based — Select this option to enable Slope-based peak detection. When enabled, peaks will be recorded if the slope algorithm indicates a peak within the Peak Width setting. 4-40
Section 4 User Help Reference • Peak Width — Select the average peak width setting. Peak widths are measured at the baseline. The slope detector will detect peak widths ranging from about 0.2 to 2 times the peak width setting. For example, if you entered a peak width of 1 minute, the range would be 12 seconds to 2 minutes. For best operation, the peak width should be set to just over the average peak width being separated. For instance, if the average peak width is 45 seconds, you should enter a peak width of 1 minute. For most flash chromatography, 1 minute is a good starting point for a peak width. • Threshold Detection — Select this option to enable Threshold peak detection. When enabled, peaks will be recorded if the Absorbance Units (AU) value is exceeded. • Threshold — Type or select the Absorbance Units value to be used for Threshold detection. Note: If both Slope and Threshold peak detection methods are checked, PeakTrak considers a peak to be present when any one condition is met. This logical OR operator means that PeakTrak will cut a peak when either the Slope condition is true, or when the Threshold condition is true. • All Wavelength Detection — Enable this option to detect peaks within a user-selected range of wavelengths. When enabled, click the Details button to configure additional settings. These settings include the slope-based and peak width options described in 1 and 2 above. Additionally, you can type or select the minimum and maximum wavelengths limits in nanometers. • External Detector — Enable this option to use a 0 to 1 volt analog input signal from an external detector. When enabled, click the Details button to configure additional settings. These settings include the slope-based and peak width options described in 1 and 2 above. PeakTrak will use its internal algorithms to cut detect and cut peaks based on the analog input signal. Refer to 4-41
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For indoor use only CombiFlash Torr
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Table of Contents 4.2 PeakTrak Menu
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