CombiFlash Torrent® User Manual - Isco
CombiFlash Torrent® User Manual - Isco
CombiFlash Torrent® User Manual - Isco
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Section 4 <strong>User</strong> Help Reference<br />
• RediSep Rf 3 kg = 4.8 L<br />
Other media types will have similar column volumes. Refer to<br />
the REDISEP RF COLUMN SELECTION GUIDE [ON-LINE] for actual<br />
volumes.<br />
As an example, consider a RediSep Rf 120 g column and a 3<br />
column volumes setting. Before instructing you to inject the<br />
sample, the module will pump 576 mL of solvent A through<br />
the column and out the waste port.<br />
Initial Waste & Time Windows The initial waste diverts a<br />
user-determined line volume to the waste port until the eluant<br />
is about to be collected. At the start of a run, the internal<br />
solvent lines hold some solvent from the previous run. This<br />
volume can be diverted to waste, along with the anticipated<br />
volume of fluid that will pass through the column before a<br />
compound of interest will elute.<br />
Time Windows can limit the fraction collection to specific time<br />
durations of the run. To use time windows enter a start time<br />
and an end time. All times are relative to start of the run, just<br />
following the sample injection. Then choose a peak collection<br />
mode. If you select Peaks the module will collect fluid only<br />
when the time window is active and a peak is present. If you<br />
select All the module will collect all fluid during the time<br />
window regardless of the peak state. Define up to three time<br />
windows.<br />
Air Purge You can program the module to purge the column<br />
with air after a run. This feature allows you to remove<br />
solvents from the column before it is discarded. Enter the<br />
desired time to purge the column, in minutes. To disable the<br />
post-run air purge, enter zero minutes.<br />
Peak Collection There are three peak collection options: All,<br />
Peaks, or None. The All option will collect peak and non-peak<br />
fluid in tubes. The Peaks option will collect only peak fluid in<br />
tubes and divert all other fluids to the waste port. The None<br />
option will divert all fluids, peak and non-peak, to the waste<br />
port. The None option is useful for developing custom methods<br />
that perform a column wash, system cleaning, or similar<br />
function where solvents and elute do not need to be collected.<br />
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